Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of SIRT3 antibody in Y79 cell line lysates (35 ug/lane). SIRT3 (arrow) was detected using the purified antibody.
Sirt3 Antibody (G265) western blot of mouse kidney tissue lysates (35 ug/lane). The Sirt3 antibody detected the Sirt3 protein (arrow).
Western blot of lysates from 293, HepG2 cell line (from left to right), using SIRT3 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
SIRT3 Antibody western blot of 293 cell lysate (35 ug/lane). This demonstrates that the SIRT3 antibody detected SIRT3 protein (arrow).
SIRT3 Antibody western blot of HepG2 cell line lysates (35 ug/lane). The SIRT3 antibody detected the SIRT3 protein (arrow).
SIRT3 Antibody western blot of KG-1 cell line lysates (35 ug/lane). The SIRT3 antibody detected the SIRT3 protein (arrow).