Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence ® PTEN siRNA I (+), using PTEN Antibody #9552 and p42 MAPK (Erk2) Antibody #9108. PTEN Antibody confirms silencing of PTEN expression, while the p42 MAPK (Erk2) Antibody is used to control for loading and specificity of PTEN siRNA.
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence ® PTEN siRNA II (+), using PTEN (138G6) Rabbit mAb and β-Actin (13E5) Rabbit mAb #4970. PTEN (138G6) Rabbit mAb confirms silencing of PTEN expression, while the β-Actin (13E5) Rabbit mAb is used to control for loading and specificity of PTEN siRNA
Western blot analysis of extracts from various cell lines, using PTEN Antibody.