PTEN

Gene Symbol PTEN
Entrez Gene 5728
Alt Symbol 10q23del, BZS, CWS1, DEC, GLM2, MHAM, MMAC1, PTEN1, TEP1
Species Human
Gene Type protein-coding
Description phosphatase and tensin homolog
Other Description MMAC1 phosphatase and tensin homolog deleted on chromosome 10|mitochondrial PTENalpha|mitochondrial phosphatase and tensin protein alpha|mutated in multiple advanced cancers 1|phosphatase and tensin-like protein|phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN|phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Swissprots Q6ICT7 B2R904 F2YHV0 P60484
Accessions AAC08699 AAD13528 AAD38372 AAY57327 ADM26753 ADM26754 ADM26755 ADM26756 ADM26759 AEZ67429 AEZ67430 AEZ67431 AEZ67432 AEZ67433 AEZ67434 AEZ67435 AEZ67436 AEZ67437 AEZ67438 BAA24090 CCF23296 CCV19970 CCV66764 CDI44160 EAW50172 EAW50173 EAW50174 EAW50175 P60484 AA017584 AA836562 AI825848 AK021619 AK024986 AK124525 AK130076 AK313581 BAG36351 BC005821 AAH05821 BG772190 BK008756 DAA64601 CA944942 CR450306 CAG29302 DQ892604 ABM83530 DQ895562 ABM86488 EU176713 ABW03514 HG503124 JF268690 ADZ48535 KJ534922 AHW56562 U92436 AAC51182 U93051 AAC51183 U96180 AAB66902 XM_006717926 XP_006717989 XM_011539981 XP_011538283 XM_011539982 XP_011538284 XR_945789 XR_945790 XR_945791 NM_000314 NP_000305 NM_001304717 NP_001291646 NM_001304718 NP_001291647
Function Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine- phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3- phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K- AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator o
Subcellular Location Cytoplasm. Nucleus. Nucleus, PML body. Note=Monoubiquitinated form is nuclear. Nonubiquitinated form is cytoplasmic. Colocalized with PML and USP7 in PML nuclear bodies. XIAP/BIRC4 promotes its nuclear localization.
Tissue Specificity Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas. {ECO:0000269|PubMed:9090379}.
Top Pathways PI3K-Akt signaling pathway, Sphingolipid signaling pathway, MicroRNAs in cancer, Central carbon metabolism in cancer, Prostate cancer

PTEN (D4.3) XP ® Rabbit mAb (Biotinylated) - 4005 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4.3
Western blot analysis of extracts from various cell lines using PTEN (D4.3) XP ® Rabbit mAb (Biotinylated).

PTEN (138G6) Rabbit mAb (Biotinylated) - 9583 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 138G6
Western Blot analysis of extracts from various cell lines using PTEN (138G6) Rabbit mAb (Biotinylated).

PTEN (138G6) Rabbit mAb (Alexa Fluor® 647 Conjugate) - 9573 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone 138G6

PTEN (138G6) Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 9560 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone 138G6

Phospho-PTEN (Ser380) Antibody - 9551 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines, using Phospho-PTEN (Ser380) Antibody (upper) or PTEN Antibody #9552 (lower). The phospho-specificity of the antibody was confirmed by treating the membrane with calf intestinal alkaline phosphatase (CIP) after Western transfer.

Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb - 9549 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone 44A7
Western blot analysis of extracts from HeLa and NIH/3T3 cells, using Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb. Membranes were either left untreated (-) or treated with (+) calf intestinal phosphatase (CIP) post Western transfer to verify phospho-specificity of the antibody.

Phospho-PTEN (Ser380/Thr382/383) Antibody - 9554 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines, using Phospho-PTEN (Ser380/Thr382/Thr383) Antibody (upper) or PTEN Antibody #9552 (lower). The phospho-specificity of the antibody was confirmed by treating the membrane with calf intestinal alkaline phosphatase (CIP) after Western transfer.

PTEN (D4.3) XP ® Rabbit mAb - 9188 from Cell Signaling Technology

Prices $109.00, $277.00, $658.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4.3
Western blot analysis of extracts from various cell lines using PTEN (D4.3) XP ® Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

PTEN (D5G7) Rabbit mAb - 5384 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5G7
Western blot analysis of extracts from HeLa and PC-3 cells using PTEN (D5G7) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower).

Non-phospho PTEN (Ser380/Thr382/Thr383) (D2D11) Rabbit mAb - 7960 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2D11
Western blot analysis of extracts from various cell lines using Non-phospho PTEN (Ser380/Thr382/Thr383) (D2D11) Rabbit mAb. The non-phosphospecificity of the antibody was verified by preincubating the antibody without peptide (-), with PTEN (Ser380/Thr382/Thr383) non-phosphopeptide (+), or with PTEN (Ser380/Thr382/Thr383) phosphopeptide (+) prior to incubating the membrane.

PTEN Antibody - 9552 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence ® PTEN siRNA I (+), using PTEN Antibody #9552 and p42 MAPK (Erk2) Antibody #9108. PTEN Antibody confirms silencing of PTEN expression, while the p42 MAPK (Erk2) Antibody is used to control for loading and specificity of PTEN siRNA.

PTEN (138G6) Rabbit mAb - 9559 from Cell Signaling Technology

Prices $99.00, $256.00, $637.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone 138G6
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence ® PTEN siRNA I (+), using PTEN Antibody #9552 and p42 MAPK (Erk2) Antibody #9108. PTEN Antibody confirms silencing of PTEN expression, while the p42 MAPK (Erk2) Antibody is used to control for loading and specificity of PTEN siRNA.

PTEN (26H9) Mouse mAb - 9556 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 26H9
Western blot analysis of extracts from various cell lines grown in media with 10% FBS, using PTEN (26H9) Mouse mAb.

Non-phospho PTEN (Ser380/Thr382/Thr383) Antibody - 9569 from Cell Signaling Technology

Prices $279.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, λ phosphatase- or PDGF-treated, using Non-phospho PTEN (Ser380/Thr382/Thr383) Antibody (upper) or PTEN (138G6) Rabbit mAb #9559 (lower). The non-phospho-specificity of the antibody was verified by preincubating the antibody with no peptide, with PTEN (Ser380/Thr382/Thr383) non-phosphopeptide or with PTEN (Ser380/Thr382/Thr383) phosphopeptide prior to incubating the membrane.

PTEN (A2B1) - sc-7974 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PTEN siRNA (h): sc-29459. Western blot analysis of PTEN expression in non-transfected control (A) and PTEN siRNA transfected (B) HeLa cells. Blot probed with PTEN (A2B1): sc-7974. Lamin A/C (H-110): sc-20681 used as specificity and loading control.

p-PTEN (H-3) - sc-377573 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of PTEN phosphorylation in non-transfected: sc-117752 (A,D), untreated mouse PTEN transfected: sc-122834 (B,E) and lambda protein phosphatase (sc-200312A) treated mouse PTEN transfected: sc-122834 (C,F) 293T whole cell lysates. Antibodies tested include p-PTEN (H-3): sc-377573 (A,B,C) and PTEN (N-19): sc-6818 (D,E,F).

PTEN (H-3) - sc-393088 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PTEN (H-3): sc-393088. Western blot analysis of PTEN expression in non-transfected: sc-117752 (A) and mouse PTEN transfected: sc-122834 (B) 293T whole cell lysates.

PTEN (F-1) - sc-393186 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PTEN (F-1): sc-393186. Western blot analysis of PTEN expression in non-transfected 293T: sc-117752 (A), mouse PTEN transfected 293T: sc-122834 (B), WI-38 (C) and MCF7 (D) whole cell lysates and human hippocampus tissue extract (E).

PTEN (B-1) - sc-133197 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PTEN (B-1): sc-133197. Western blot analysis of PTEN expression in non-transfected: sc-117752 (A) and mouse PTEN transfected: sc-122834 (B) 293T whole cell lysates.

PTEN (DCS-202) - sc-56204 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

PTEN (FL-403) - sc-9145 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PTEN (FL-403): sc-9145. Western blot analysis of human recombinant PTEN fusion protein.

PTEN (N-19) - sc-6818 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PTEN (N-19): sc-6818. Western blot analysis of PTEN expression in WI 38 whole cell lysate.

PTEN (10P01) - sc-73420 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

PTEN (G-6) - sc-133242 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PTEN (G-6): sc-133242. Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing cytoplasmic staining of urothelial cells.

PTEN (SPM440) - sc-65604 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
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