Anti-SMAD2 Antibody (Thr220) IHC-plusâ¢
| Name | Anti-SMAD2 Antibody (Thr220) IHC-plus⢠|
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-B9109 |
| Prices | $395.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC-P WB |
| Species Reactivities | Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken, Xenopus |
| Purity/Format | Protein A purified |
| Blocking Peptide | SLU7 / 9G8 Antibody Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | SMAD2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Fluorescent confocal image of SY5Y cells stained with SMAD2 (T220) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated SMAD2 (T220) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the SMAD2 mainly to the nucleus.
Western blot of SMAD2 Antibody (T220) in NCI-H460 cell line lysates (35 ug/lane). SMAD2 (arrow) was detected using the purified antibody.