SMAD2

Gene Symbol SMAD2
Entrez Gene 4087
Alt Symbol JV18, JV18-1, MADH2, MADR2, hMAD-2, hSMAD2
Species Human
Gene Type protein-coding
Description SMAD family member 2
Other Description MAD homolog 2|SMAD, mothers against DPP homolog 2|Sma- and Mad-related protein 2|mother against DPP homolog 2|mothers against decapentaplegic homolog 2
Swissprots Q15796
Accessions AAC39657 EAW62914 EAW62915 EAW62916 EAW62917 Q15796 AA081871 AA418737 AF027964 AAC51918 AI087928 AI453799 AI765747 AK126373 AK299218 BAH12971 AK312963 BAG35802 AY134745 AAN08619 BC014840 AAH14840 BC025699 AAH25699 BI258844 BQ018815 BQ900993 BT007422 AAP36090 BU167620 BU517178 BX491944 CR992221 DB035686 DB462454 DC371139 DQ891501 ABM82427 DQ893532 ABM84458 DQ893746 ABM84672 DQ894693 ABM85619 U59911 AAC50789 U65019 AAB17054 U68018 AAB17087 XM_005258259 XP_005258316 XM_006722451 XP_006722514 XM_011525983 XP_011524285 XM_011525984 XP_011524286 XM_011525985 XP_011524287 XM_011525986 XP_011524288 NM_001003652 NP_001003652 NM_001135937 NP_001129409 NM_005901 NP_005892
Function Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. {ECO:0000269|PubMed:16751101, ECO:0000269|PubMed:16862174, ECO:0000269|PubMed:17327236, ECO:0000269|PubMed:19289081, ECO:0000269|PubMed:9892009}.
Subcellular Location Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1.
Tissue Specificity Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta. {ECO:0000269|PubMed:21599657}.
Top Pathways Chagas disease (American trypanosomiasis), Inflammatory bowel disease (IBD), Signaling pathways regulating pluripotency of stem cells, Proteoglycans in cancer, Hippo signaling pathway

Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb (PE Conjugate) - 11979 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D27F4
Flow cytometric analysis of HT-1080 cells, treated with hTGF-β3 #8425 (green) or hTGF-β3 and SB431542 (blue), using Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb (PE Conjugate).

Smad2 (D43B4) XP ® Rabbit mAb (Biotinylated) - 12584 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D43B4
Western blot analysis of extracts from HeLa and C2C12 cells using Smad2 (D43B4) XP ® Rabbit mAb (Biotinylated).

Smad2/3 (D7G7) XP ® Rabbit mAb (Biotinylated) - 12470 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D7G7
Western blot analysis of extracts from HeLa and C2C12 cells using Smad2/3 (D7G7) XP ® Rabbit mAb (Biotinylated). Streptavidin-HRP #3999 was used for western detection.

Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb - 8828 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D27F4
Western blot analysis of extracts from HaCaT cells, untreated (-) or treated with hTGF-β3 #8425 (+) in the absence or presence of the TGFR inhibitor SB 431542, using Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) (D27F4) Rabbit mAb (upper) or Smad2/3 (D7G7) XP ® Rabbit mAb #8685 (lower).

Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb - 3108 from Cell Signaling Technology

Prices $109.00, $307.00, $719.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Monoclonal
Clone 138D4
Western blot analysis of extracts from untreated or TGF-beta treated HeLa and NIH/3T3 cells, using Phospho-Smad2 (Ser465/467) (138D4) Rabbit mAb (upper), or Smad2 Antibody #3102 (lower).

Phospho-Smad2 (Ser465/467) Antibody - 3101 from Cell Signaling Technology

Prices $279.00, $659.00
Sizes 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HepG2 cells treated with TGFbeta for the indicated times, using Phospho-Smad2 (Ser465/467) Antibody (upper) or Smad2 antibody (lower).

Phospho-Smad2 (Ser245/250/255) Antibody - 3104 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293 and HeLa cells, untreated or TPA-treated (200 nM for 30 minutes) using Phospho-Smad2 (Ser245/250/255) Antibody (upper) or Smad2 Antibody (lower).

Smad2 (D43B4) XP ® Rabbit mAb - 5339 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D43B4
Western blot analysis of extracts from various cell lines using Smad2 (D43B4) XP ® Rabbit mAb.

Smad2/3 (D7G7) XP ® Rabbit mAb - 8685 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D7G7
Western blot analysis of extracts from HeLa and ACHN cells using Smad2/3 (D7G7) XP ® Rabbit mAb.

Smad2 (86F7) Rabbit mAb - 3122 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 86F7
Western blot analysis of extracts from HeLa, H1975 and HT29 cell lines, using Smad2 (86F7) Rabbit mAb.

Smad2/3 Antibody - 5678 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from ACHN and HeLa cells using Smad2/3 Antibody.

Smad2 (L16D3) Mouse mAb - 3103 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone L16D3
Western blot analysis of extracts from HeLa, NIH/3T3, COS, and PC12 cells, using Smad2 (L16D3) Mouse mAb.

Smad2/3 Antibody - 3102 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of various cell lines using Smad2/3 Antibody.

Phospho-Smad2 (Ser465/467)/ Smad3 (Ser423/425) (D6G10) XP ® Rabbit mAb - 9510 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone D6G10

Smad2 (34G6) Mouse mAb - 3107 from Cell Signaling Technology

Host Mouse
Clonality Monoclonal
Clone 34G6

Smad2 (S-20) - sc-6200 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Smad2 (S-20): sc-6200. Western blot analysis of Smad2 expression in non-transfected: sc-117752 (A) and human Smad2 transfected: sc-114131 (B) 293T whole cell lysates.

Smad2 (A-11) - sc-393312 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Smad2 (A-11): sc-393312. Western blot analysis of Smad2 expression in non-transfected 293T: sc-117752 (A), human Smad2 transfected 293T: sc-114131 (B), K-562 (C), KNRK (D) and NIH/3T3 (E) whole cell lysates. Note lack of reactivity in lanes D and E.

Smad2 (YZ-13) - sc-101153 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Smad2 (YZ-13): sc-101153. Western blot analysis of Smad2 expression in non-transfected: sc-117752 (A) and human Smad2 transfected: sc-113452 (B) 293T whole cell lysates.

Smad2/3 (A-3) - sc-398844 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Smad2/3 (A-3): sc-398844. Western blot analysis of Smad2/3 expression in U-937 whole cell lysate.

Smad2/3 (E-20) - sc-6033 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Smad2/3 (E-20): sc-6033. Immunofluorescence staining of methanol-fixed U-937 cells showing nuclear localization.

Smad2/3 (I-20) - sc-6202 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Smad2/3 (I-20): sc-6202. Western blot analysis of Smad2/3 expression in U-937 (A) and K-562 (B) whole cell lysates.

Smad2/3 (N-19) - sc-6032 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Smad2/3 (N-19): sc-6032. Western blot analysis of Smad2/3 expression in C2C12 (A), Mv 1 Lu (B) and U-937 (C) whole cell lysates.

p-Smad2 (Ser 467) - sc-101801 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Smad2 (Ser 467): sc-101801. Western blot analysis of phosphorylated Smad2 expression in untreated (A) and PMA-treated (B) 293 whole cell lysates.

p-Smad2 (Thr 220) - sc-135644 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Smad2 (Thr 220): sc-135644. Western blot analysis of phosphorylated Smad2 expression in untreated (A) and IL-4-treated (B) HeLa cell extracts.

Anti-Smad2 + Smad3 (phospho T8) antibody - ab63399 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Smad2 + Smad3 (phospho T8) antibody (ab63399) at 1/500 dilutionLane 1 : Extracts from RAW264.7 cellsLane 2 : Extracts from RAW264.7 cells plus phospho peptideObserved band size : 48 kDa (why is the actual band size different from the predicted?)
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