Detection of Human SMC5 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-SMC5 antibody used for IP at 6 ug/mg lysate. SMC5 was also immunoprecipitated by a prior lot (-1) of this antibody. For blotting immunoprecipitated SMC5, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human and Mouse SMC5 by Western Blot. Samples: Whole cell lysate (50 ug) from 293T, HeLa, Jurkat, TCMK-1 and NIH3T3 cells. Antibodies: Affinity purified rabbit anti-SMC5 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.