Western blot analysis of extracts from RAW, THP1 and p388D1 cells using PU.1 (9G7) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic mouse tumor using PU.1 (9G7) Rabbit mAb #2258.
Immunohistochemical analysis of paraffin-embedded Non-Hodgkin's lymphoma using PU.1 (9G7) Rabbit mAb in the presence of control peptide (left) or PU.1 Blocking Peptide #1036 (right).
Confocal immunofluorescent analysis of Raw cells using PU.1 (9G7) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
Flow cytometric analysis of MCF7 cells (blue) and THP1 cells (green) using PU.1 (9G7) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 U-937 cells and either 5 μl of PU.1 (9G7) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human CD11b promoter primers, human CD18 intron 1 primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.