p-Op18 (Ser 16)-R: sc-12948-R. Western blot detection of Op18 phosphorylation in Jurkat cells. Antibody was preincubated with cognate non-phosphorylated (A) or phosphorylated peptide (B).
p-Op18 (Ser 16): sc-12948. Immunofluorescence staining of methanol-fixed Jurkat cells showing cytoplasmic localization.
p-Op18 (Ser 16)-R: sc-12948-R. Western blot analysis of Op18 (Ser 16) phosphorylation in untreated (A), PMA-treated (B) and PMA and lambda protein phosphatase treated (C) Jurkat whole cell lystates.
Western blot analysis of Op18 phosphorylation in untreated (A,D), PMA treated (B,E) and PMA and lambda protein phosphatase treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-Op18 (Ser 16)-R: sc-12948-R (A,B,C) and Op18 (FL-149): sc-20796 (D,E,F).
Western blot analysis of Op18 phosphorylation in untreated (A,C) and activator cocktail (sc-362324) treated (B,D) Jurkat whole cell lysates. Antibodies tested include p-Op18 (Ser 16)-R: sc-12948-R (A,B) and Op18 (A-4): sc-48362 (C,D).