Formalin-fixed and paraffin-embedded human Spleen tissue reacted with SNAIL antibody (N-term D24) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
HepG2 cells were incubated with HGF for the time periods indicated. LiCl and MG132 were added 8 h before lysis of the cells. Snail protein levels and beta actin as loading control were analyzed by WB.
Western blot of SNAIL (arrow) using rabbit polyclonal hSNAIL-D24. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SNAIL gene (Lane 2).