Detection of Human U1C by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-U1C antibody used for IP at 6 ug/mg lysate. For blotting immunoprecipitated U1C, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Mouse U1C by Western Blot. Samples: Whole cell lysate (50 ug) from Renca and TCMK-1 cells. Antibodies: Affinity purified rabbit anti-U1C antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human U1C by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 ug), and 293T (50 ug) cells. Antibodies: Affinity purified rabbit anti-U1C antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.