
Western blot analysis of extracts from various cell lines using Rab25 (D4P6P) XP ® Rabbit mAb.

Western blot analysis of extracts from MCF7 (positive) and HeLa (negative) cells using Rab25 (D4P6P) XP ® Rabbit mAb and β-Actin (D6A8) Rabbit mAb #8457 as a loading control.

Immunoprecipitation of Rab25 from ZR-75-1 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Rab25 (D4P6P) XP ® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Rab25 (D4P6P) XP ® Rabbit mAb.

Confocal immunofluorescent analysis of MCF7 (positive, left) and HeLa (negative, right) cells using Rab25 (D4P6P) XP ® Rabbit mAb (green). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).