Formalin-fixed and paraffin-embedded human hepatocarcinoma with Phospho-SRC-Y419.control, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent of SRC Antibody (Y419) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Western blot of lysate from A375 cell line, using SRC Antibody (Y419). Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
Western blot of Phospho-SRC-Y419 in HepG2 cell line lysates (35 ug/lane). SRC (arrow) was detected using the purified antibody.