Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-Phospho-STAT5a-S726 Antibody in CEM cell line lysate (35 ug/lane). Phospho-STAT5a(arrow) was detected using the purified antibody.
Dot blot of anti-Phospho-hSTAT5a-pS726 Phospho-specific Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.