Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of lysates from A431, K562, CEM cell line and rat brain tissue lysate(from left to right), using SUV39H2 Antibody (K315). Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Western blot of anti-hSUV39H2-K315 antibody in 293 and HL60 cell line lysates (35 ug/lane). hSUV39H2-K315(arrow) was detected using the purified antibody.