Western blot analysis of extracts from 293T cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-) or SignalSilence ® RAP80 siRNA #14519 (+), using RAP80 (D1T6Q) Rabbit mAb (upper) and α-Actinin (D6F6) XP ® Rabbit mAb #6487 (lower).
Western blot analysis of extracts from various cell lines using RAP80 (D1T6Q) Rabbit mAb.
Confocal immunofluorescent analysis of 293T cells either untreated (left) or neocarzinostatin-treated (5 μg/ml; 1hr, right) using RAP80 (D1T6Q) Rabbit mAb (green). RAP80 foci become more numerous with neocarzinostatin treatment. Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red).