Western blot analysis of extracts from WI-38 cells, serum-starved for three days and serum-stimulated for the indicated times, using Rb (D20) Rabbit mAb.
Immunoprecipitation of Rb from Jurkat cells using Rb (D20) Rabbit mAb. Western blot was performed using the same antibody.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 Raji cells and either 5 μl of Rb (D20) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using using SimpleChIP ® Human Timeless Intron 1 Primers #7001, human DHFR promoter primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.