RB1

Gene Symbol RB1
Entrez Gene 5925
Alt Symbol OSRC, PPP1R130, RB, p105-Rb, pRb, pp110
Species Human
Gene Type protein-coding
Description retinoblastoma 1
Other Description prepro-retinoblastoma-associated protein|protein phosphatase 1, regulatory subunit 130|retinoblastoma suspectibility protein|retinoblastoma-associated protein
Swissprots Q8IZL4 P78499 A8K5E3 P06400 Q5VW46
Accessions AAA53483 AAA53484 AAA65735 AAA65736 AAA65737 AAA65738 AAA65739 AAA65740 AAA65741 AAA65742 AAA65743 AAA65744 AAA65745 AAA65746 AAA65747 AAA65748 AAA65749 AAA65998 AAA82538 AAA82539 AAA82540 AAA82541 AAA82542 AAA82543 AAA82544 AAA82545 AAA82546 AAA82547 AAA82548 AAA82549 AAA82550 AAA82551 AAA82552 AAA82553 AAA82554 AAA82555 AAA82556 AAA82557 AAA82558 AAA82559 AAA82560 AAA82561 AAA82562 AAA82563 AAB59466 AAB59467 AAB59468 AAB59469 AAB59470 AAB59471 AAB59472 AAB59473 AAB59474 AAB59475 AAB59476 AAB59477 AAB59478 AAB59479 AAB59480 AAB59481 AAB59482 AAB59483 AAB59489 AAB59579 AAB59580 AAN41646 AAN64133 AAO62758 AAP39867 AAT66036 AAT77205 AAT77206 AAT77207 AAV84025 ABC47164 CAA34462 EAX08793 EAX08794 P06400 AB208788 BAD92025 AF043224 AAC26845 AK291258 BAF83947 AK299179 BAG61225 AK300284 BAG62042 AK307125 AK307730 AY429568 BC039060 AAH39060 BC040540 AAH40540 DQ891564 ABM82490 DQ894752 ABM85678 L41870 AAB59465 M15400 AAA69807 M19701 AAA60259 M28419 AAA69808 M33647 AAA69806 XM_011535171 XP_011533473 NM_000321 NP_000312
Function Key regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor compl
Subcellular Location Nucleus {ECO:0000269|PubMed:20940255}.
Tissue Specificity Expressed in the retina.
Top Pathways Cell cycle, Prostate cancer, Glioma, Pancreatic cancer, Non-small cell lung cancer

Phospho-Rb (Ser807/811) (D20B12) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 4277 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D20B12
Confocal immunofluorescent analysis of untreated MCF7 (left), λ phosphatase-treated MCF7 (middle), and BT-549 (right) cells using Phospho-Rb (Ser807/Ser811) (D20B12) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red).

Phospho-Rb (Ser807/811) (D20B12) XP ® Rabbit mAb (PE Conjugate) - 11917 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D20B12
Flow cytometric analysis of BT-549 (blue) and Jurkat (green) cells using Phospho-Rb (Ser807/811) (D20B12) XP ® Rabbit mAb (PE Conjugate).

Phospho-Rb (Ser807/811) (D20B12) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 8974 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D20B12
Flow cytometic analysis of BT-549 (blue) and Jurkat (green) cells using Phospho-Rb (Ser807/811) (D20B12) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate).

Phospho-Rb (Ser807/811) (D20B12) XP ® Rabbit mAb (Alexa Fluor ® 594 Conjugate) - 8880 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D20B12
Confocal immunofluorescent analysis of MCF7 cells, untreated (left) or λ phosphatase-treated (middle), and BT-549 cells (right), using Phospho-Rb (Ser807/811) (D20B12) XP ® Rabbit mAb (Alexa Fluor ® 594 Conjugate) (red). Actin filaments were labeled with Alexa Fluor ® 488 phalloidin (green).

Phospho-Rb (Ser807/811) (D20B12) XP ® Rabbit mAb (Alexa Fluor ® 555 Conjugate) - 8957 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D20B12
Confocal immunofluorescent analysis of MCF7 cells, untreated (left) or λ phosphatase-treated (middle), and BT-549 cells (right), using Phospho-Rb (Ser807/811) (D20B12) XP ® Rabbit mAb (Alexa Fluor ® 555 Conjugate) (red). Actin filaments were labeled with Alexa Fluor ® 488 phalloidin (green).

Rb (4H1) Mouse mAb (PE Conjugate) - 14731 from Cell Signaling Technology

Prices $299.00
Sizes 100 µl (50 tests)
Host Mouse
Clonality Monoclonal
Clone 4H1
Flow cytometric analysis of Jurkat cells using Rb (4H1) Mouse mAb (PE Conjugate) and DRAQ5 ® #4084 to measure DNA content.

Phospho-Rb (Ser807/811) (D20B12) XP ® Rabbit mAb - 8516 from Cell Signaling Technology

Prices $119.00, $318.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D20B12
Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with calf intestinal phosphatase (CIP) and λ phosphatase (+), using Phospho-Rb (Ser807/811) (D20B12) XP ® Rabbit mAb (upper) or Rb (4H1) Mouse mAb #9309 (lower).

PhosphoPlus® Rb (Ser780, Ser807/811) Antibody Kit - 9306 from Cell Signaling Technology

Prices $489.00
Sizes 1 kit (10 western blots)
Clonality Polyclonal

Phospho-Rb (Ser608) (D10F2) Rabbit mAb - 8147 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D10F2
Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with calf intestinal phosphatase (CIP) and λ phosphatase (+), using Phospho-Rb (Ser608) (D10F2) Rabbit mAb (upper) or Rb (D20) Rabbit mAb #9313 (lower).

Phospho-Rb (Ser780) Antibody - 9307 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from human fibroblasts synchronized by serum deprivation, using Phospho-Rb (Ser780) Antibody. Cells were synchronized for 24 hours then released by addition of serum and harvested at the times indicated. Cell cycle progression was verified by cyclin analysis and FACS. (Provided by John Boylan, Dupont/Merck, Delaware.)

Phospho-Rb (Ser780) (D59B7) Rabbit mAb - 8180 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D59B7
Western blot analysis of extracts from WI-38 cells, serum-starved for 3 days (-) or serum-starved for 3 days followed by treatment with 10% serum for 2 days (+), using Phospho-Rb (Ser780) (D59B7) Rabbit mAb.

Phospho-Rb (Ser807/811) Antibody - 9308 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from human fibroblasts synchronized by serum deprivation, using Phospho-Rb (Ser807/811) Antibody. Cells were synchronized for 24 hours then released by addition of serum and harvested at the times indicated. Cell cycle progression was verified by cyclin analysis and FACS. (Provided by John Boylan, Dupont/Merck, Delaware.)

Phospho-Rb (Ser780) (C84F6) Rabbit mAb - 3590 from Cell Signaling Technology

Prices $297.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C84F6
Western blot analysis of recombinant truncated Rb, without or with Rb blocking peptide, using Phospho-Rb (Ser780) (C84F6) Rabbit mAb.

Phospho-Rb (Ser608) Antibody - 2181 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells treated with lambda Phosphatase NEB #P0753 (10,000 units/ml, 1 h) or calyculin A #9902 (100 nM, 10 min) using Phospho-Rb (Ser608) Antibody (upper) or Rb (4H1) Mouse mAb #9309 (lower).

Phospho-Rb (Ser795) Antibody - 9301 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from human fibroblasts synchronized by serum deprivation, using Phospho-Rb (Ser795) Antibody. Cells were synchronized for 24 hours, then released by addition of serum and harvested at the times indicated. Cell cycle progression was verified by cyclin analysis and FACS. (Provided by John Boylan, Dupont/Merck, Delaware.)

Rb (D20) Rabbit mAb - 9313 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D20
Western blot analysis of extracts from WI-38 cells, serum-starved for three days and serum-stimulated for the indicated times, using Rb (D20) Rabbit mAb.

Rb (4H1) Mouse mAb - 9309 from Cell Signaling Technology

Prices $99.00, $246.00, $596.00
Sizes 20 µl (2 western blots), 100 µl (20 western blots), 300 µl (60 western blots)
Host Mouse
Clonality Monoclonal
Clone 4H1
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence ® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence ® Rb siRNA I #6451 (+) or SignalSilence ® Rb siRNA II (+), using Rb (4H1) Mouse mAb #9309 and α-Tubulin (11H10) Rabbit mAb #2125. The Rb (4H1) Mouse mAb confirms silencing of Rb expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Rb siRNA.

PhosphoPlus® Rb (Ser780, Ser795, Ser807/811) Antibody Kit - 9300 from Cell Signaling Technology

Clonality Polyclonal
Western blot analysis of extracts from human fibroblasts synchronized by serum deprivation, using Phospho-Rb (Ser795) Antibody. Cells were synchronized for 24 hours, then released by addition of serum and harvested at the times indicated. Cell cycle progression was verified by cyclin analysis and FACS. (Provided by John Boylan, Dupont/Merck, Delaware.)

Phospho-Rb (Ser780) Antibody - 5225 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Rb (D3D2) XP ® Rabbit mAb - 9305 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone D3D2
Western blot analysis of extracts from MCF-7, HCT-116 and BT-549 cells using Rb (D3D2) XP ® Rabbit mAb. BT-549 cells do not express Rb.

Rb (4H1) Mouse mAb - 5230 from Cell Signaling Technology

Host Mouse
Clonality Monoclonal
Clone 4H1

Rb Antibody - 9302 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Rb (H-2) - sc-74570 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rb (H-2): sc-74570. Western blot analysis of Rb expression in non-transfected 293T: sc-117752 (A), human Rb transfected 293T: sc-114014 (B) and K-562 (C) whole cell lysates.

Rb (IF8) - sc-102 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rb (IF8): sc-102. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing nuclear staining.

Rb (Rb1) - sc-73598 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Rb (Rb1): sc-73598. Western blot analysis of Rb expression in K-562 (A), A-431 (B), HeLa (C) and SK-LMS-1 (D) whole cell lysates.
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