Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 Jurkat cells treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (50ng/ml) and Ionomycin, Calcium Salt #9995 (200ng/ml) and either 5 μl of c-Rel (D5G1A) Rabbit mAb (ChIP Formulated) or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human BCL2L1 promoter primers, SimpleChIP ® Human EP300 Promoter Primers #12281, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.