Formalin-fixed and paraffin-embedded human breast carcinoma reacted with TIMP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescent of TIMP1 Antibody with A2058 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Western blot of TIMP1 Antibody in CEM cell line lysates (35 ug/lane). TIMP1 (arrow) was detected using the purified antibody.
Flow cytometric of MDA-231 cells using TIMP1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.