Anti-TIP30 / HTATIP2 antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10900 concentration 5 ug/ml.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TIP30 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.
Sample (30 ug of whole cell lysate) A: A431 B: HeLa 12% SDS PAGE HTATIP2 / TIP30 antibody diluted at 1:5000
IP Sample: hela whole cell lysate/extract A. 40 µg hela whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of TIP30 protein by 2 µg of TIP30 antibody 10% SDS-PAGE The immunoprecipitated TIP30 protein was detected by TIP30 antibody diluted at 1:1000.