Western blot analysis of extracts from various cell lines and tissues using Rev-Erbα (E1Y6D) Rabbit mAb.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc-tagged full length human Rev-erbα (hRev-erbα-Myc; +), using Rev-Erbα (E1Y6D) Rabbit mAb.
Immunoprecipitation of Rev-erbα from Hep G2 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Rev-Erbα (E1Y6D) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Rev-Erbα (E1Y6D) Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 Hep G2 cells and either 10 μl of Rev-Erbα (E1Y6D) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human BMAL1 promoter primers, SimpleChIP ® Human NR1D1 Promoter Primers #13413, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.