Phospho-RIP2 (Ser176) (E1I9J) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 14397
• Prices: $287.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: E1I9J
• Applications: WB IP
• Species Reactivities: Human
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser176 of human RIP2 protein.
• Description: Rabbit Monoclonal
• Gene: RIPK2

Product images

Western blot analysis of extracts from THP-1 cells, differentiated with TPA #4174 (80 nM, 24 hr) followed by treatment with LPS (1 μg/ml) for the indicated times, using Phospho-RIP2 (Ser176) (E1I9J) Rabbit mAb (upper) and RIP2 (D10B11) Rabbit mAb #4142 (lower).

Western blot analysis of extracts from OVCAR8 cells, untreated (-) or UV-treated (+), using Phospho-RIP2 (Ser176) (E1I9J) Rabbit mAb (upper) and RIP2 (D10B11) Rabbit mAb #4142 (lower).

Immunoprecipitation of phospho-RIP2 (Ser176) from THP-1 cells, treated with TPA #4174 (80 nM, 24 hr) followed by LPS (1 μg/ml, 15 min), using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Phospho-RIP2 (Ser176) (E1I9J) Rabbit mAb (lane 3). Lane 1 represents 10% input. Western blot was performed using Phospho-RIP2 (Ser176) (E1I9J) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as a secondary antibody to avoid cross reactivity with rabbit IgG.