Formalin-fixed and paraffin-embedded human Lung carcinoma reacted with ACK1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of ACK1 (arrow) using rabbit polyclonal ACK1 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ACK1 gene (Lane 2) (60 KD recombinant protein).
Western blot of ACK1 Antibody in K562 cell line lysates (35 ug/lane). ACK1 (arrow) was detected using the purified antibody.
Flow cytometric of NCI-H292 cells using ACK1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.