TNK2

Gene Symbol TNK2
Entrez Gene 10188
Alt Symbol ACK, ACK-1, ACK1, p21cdc42Hs
Species Human
Gene Type protein-coding
Description tyrosine kinase, non-receptor, 2
Other Description activated CDC42 kinase 1|activated Cdc42-associated kinase 1|activated p21cdc42Hs kinase|tyrosine kinase non-receptor protein 2
Swissprots Q96H59 Q6ZMQ0 Q07912 Q8N6U7
Accessions EAW50333 EAW50334 EAW50335 EAW50336 EAW50337 Q07912 AB209338 BAD92575 AK024904 AK126712 AK127455 BAG54506 AK131539 BAD18675 AK225786 AK309332 BC008884 AAH08884 BC020389 BC028164 AAH28164 BC156798 AAI56799 BE676668 BG742978 CN411136 DQ596952 L13738 AAA53570 XM_005269268 XP_005269325 XM_005269270 XP_005269327 XM_005269272 XP_005269329 XM_005269274 XP_005269331 XM_005269275 XP_005269332 XM_011512317 XP_011510619 XM_011512318 XP_011510620 XM_011512319 XP_011510621 XM_011512320 XP_011510622 XM_011512321 XP_011510623 NM_001010938 NP_001010938 NM_001308046 NP_001294975 NM_005781 NP_005772
Function Non-receptor tyrosine-protein and serine/threonine- protein kinase that is implicated in cell spreading and migration, cell survival, cell growth and proliferation. Transduces extracellular signals to cytosolic and nuclear effectors. Phosphorylates AKT1, AR, MCF2, WASL and WWOX. Implicated in trafficking and clathrin-mediated endocytosis through binding to epidermal growth factor receptor (EGFR) and clathrin. Binds to both poly- and mono-ubiquitin and regulates ligand-induced degradation of EGFR, thereby contributing to the accumulation of EGFR at the limiting membrane of early endosomes. Downstream effector of CDC42 which mediates CDC42-dependent cell migration via phosphorylation of BCAR1. May be involved both in adult synaptic function and plasticity and in brain development. Activates AKT1 by phosphorylating it on 'Tyr-176'. Phosphorylates AR on 'Tyr-267' and 'Tyr-363' thereby promoting its recruitment to androgen-responsive enhancers (AREs). Phosphorylates WWOX on 'Tyr- 287'. Phos
Subcellular Location Cell membrane. Nucleus. Endosome. Cell junction, adherens junction {ECO:0000250}. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, clathrin-coated vesicle. Membrane, clathrin- coated pit. Note=The Tyr-284 phosphorylated form is found both in the membrane and nucleus. Colocalizes with EGFR on endosomes. Nuclear translocation is CDC42-dependent.
Tissue Specificity The Tyr-284 phosphorylated form shows a significant increase in expression in breast cancers during the progressive stages i.e. normal to hyperplasia (ADH), ductal carcinoma in situ (DCIS), invasive ductal carcinoma (IDC) and lymph node metastatic (LNMM) stages. It also shows a significant increase in expression in prostate cancers during the progressive stages. {ECO:0000269|PubMed:16247015, ECO:0000269|PubMed:20333297, ECO:0000269|PubMed:20623637}.

Phospho-Ack1 (Tyr284) Antibody - 3138 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS cells, untransfected or overexpressing Myc-tagged Ack1 protein, using Phospho-Ack1 (Tyr284) Antibody (upper and middle) and Myc-Tag (71D10) Rabbit mAb #2278 (lower). The middle blot was treated with calf intestinal phosphatase (CIP) before antibody probing. (The lysates of COS cells overexpressing Myc-tagged Ack1 were kindly provided by Dr. Wannian Yang, Weis Center for Research, Geisinger Clinic.)

Phospho-Ack1 (Tyr857/858) Antibody - 3137 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Phospho-Ack1 (Tyr857/858) (C57E10) Rabbit mAb - 3097 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone C57E10

Ack1 Antibody - 3131 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

ACK (A-11) - sc-28336 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ACK (A-11): sc-28336. Western blot analysis of ACK expression in H4 (A), MM-142 (B) and EOC 20 (C) whole cell lysates.

ACK (C-20) - sc-323 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ACK (C-20): sc-323. Immunofluorescence staining of methanol-fixed H4 cells showing cytoplasmic localization.

ACK (H-172) - sc-9165 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Western blot analysis of ACK expression in EOC 20 (A,E), MM-142 (B,D) and H4 (C) whole cell lysates. Antibodies tested include ACK (H-172): sc-9165 (A,B) and ACK (A-11): sc-28336 (C,D,E).

ACK (K-15) - sc-30695 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ACK (K-15): sc-30695. Western blot analysis of ACK expression in HeLa nuclear extract.

Anti-ACK1 (phospho Y284) antibody - ab74091 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab74091 at 1/500 dilution staining ACK1 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Anti-ACK1 antibody - ab185726 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue labelling ACK1 with ab185726 at 1/200. Magnification: 400x.

Anti-ACK1 antibody - ab135672 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab135672 stained MDA-MB-231 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab135672 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-ACK1 antibody - ab75910 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-ACK1 antibody - ab172553 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-ACK1 antibody (ab172553) at 1 µg/mlLane 1 : ACK1 transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-ACK1 antibody - ab37367 from Abcam

Prices $370.00
Sizes 50 µg
Host Chicken
Clonality Polyclonal

Anti-ACK1 antibody - C-terminal - ab135673 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-ACK1 antibody - C-terminal (ab135673) at 1/10 dilution + CEM cell line lysate at 35 µg

Anti-TNK2 / ACK1 Antibody - LS-C332144 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded rat spleen using TNK2 antibody at dilution of 1:200 (400x lens).

Anti-TNK2 / ACK1 Antibody (aa814-844) - LS-C155934 from LifeSpan Bioscience

Prices $250.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-TNK2 / ACK1 Antibody (Asn280) - LS-C193232 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-TNK2 / ACK1 Antibody (aa265-290, AP) - LS-C250669 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TNK2 / ACK1 Antibody (aa265-290, APC) - LS-C250670 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TNK2 / ACK1 Antibody (aa265-290, Biotin) - LS-C250671 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TNK2 / ACK1 Antibody (aa265-290, FITC) - LS-C250672 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TNK2 / ACK1 Antibody (aa265-290, PE) - LS-C250674 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TNK2 / ACK1 Antibody (aa265-290, Azide-free, HRP) - LS-C250673 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-TNK2 / ACK1 Antibody (phospho-Tyr518) - LS-C97107 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Dot blot of anti-Phospho-ACK1-pY518 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
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