Detection of Human GW182 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-GW182 antibody used for IP at 6 ug/mg lysate. GW182 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated GW182, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Detection of Human GW182 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-GW182 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.