TNRC6A

Gene Symbol TNRC6A
Entrez Gene 27327
Alt Symbol CAGH26, GW1, GW182, TNRC6
Species Human
Gene Type protein-coding
Description trinucleotide repeat containing 6A
Other Description CAG repeat protein 26|EDIE|EMSY interactor protein|GW182 autoantigen|glycine-tryptophan protein of 182 kDa|trinucleotide repeat-containing gene 6A protein
Swissprots Q9NV59 Q8NDV7 Q9P268 Q658L5 O15408 Q8TCR0 Q8TBT8 Q6NVB5 C9JAR8 Q8NEZ0
Accessions EAW55782 EAW55783 EAW55784 EAW55785 EAW55786 EAW55787 Q8NDV7 AB040893 BAA95984 AJ492221 CAD37348 AK001773 BAA91899 AK025277 AK025696 AK094542 BAG52884 AK299905 BAG61746 AL713750 CAD28525 AL833757 CAH56236 AY035864 AAK62026 BC024324 AAH24324 BC068209 BC172409 AAI72409 BE158958 BU743266 U23860 U80739 AAB91438 XM_005255254 XP_005255311 XM_005255257 XP_005255314 XM_006721039 XP_006721102 XM_011545791 XP_011544093 XM_011545792 XP_011544094 XM_011545793 XP_011544095 XM_011545794 XP_011544096 XM_011545795 XP_011544097 XM_011545796 XP_011544098 NM_014494 NP_055309
Function Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA- dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. As scaffoldng protein associates with argonaute proteins bound to partially complementary mRNAs and simultaneously can recruit CCR4-NOT and PAN deadenylase complexes. {ECO:0000269|PubMed:16284622, ECO:0000269|PubMed:16284623, ECO:0000269|PubMed:17596515, ECO:0000269|PubMed:17671087, ECO:0000269|PubMed:19056672, ECO:0000269|PubMed:19304925}.
Subcellular Location Cytoplasm, P-body {ECO:0000269|PubMed:11950943, ECO:0000269|PubMed:13130130, ECO:0000269|PubMed:15494374, ECO:0000269|PubMed:15908945, ECO:0000269|PubMed:16284622, ECO:0000269|PubMed:16284623, ECO:0000269|PubMed:16906129, ECO:0000269|PubMed:18946079, ECO:0000269|PubMed:19056672, ECO:0000269|PubMed:19324964}. Note=Mammalian P-bodies are also known as GW bodies (GWBs).
Tissue Specificity Ubiquitous. {ECO:0000269|PubMed:11950943}.

GW182 (A-8) - sc-377006 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GW182 (A-8): sc-377006. Western blot analysis of GW182 expression in non-transfected: sc-117752 (A) and human GW182 transfected: sc-113721 (B) 293T whole cell lysates.

GW182 (E-1) - sc-376939 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GW182 (E-1): sc-376939. Western blot analysis of GW182 expression in non-transfected: sc-117752 (A) and human GW182 transfected: sc-113721 (B) 293T whole cell lysates.

GW182 (G-10) - sc-374459 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GW182 (G-10): sc-374459. Western blot analysis of GW182 expression in non-transfected: sc-117752 (A) and human GW182 transfected: sc-113721 (B) 293T whole cell lysates.

GW182 (M-19) - sc-47037 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GW182 (M-19) : sc-47037. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of Purkinje cells, cells in granular layer and cells in molecular layer.

GW182 (A-6) - sc-374458 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GW182 (A-6): sc-374458. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.

GW182 (4B6) - sc-56314 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GW182 (4B6): sc-56314. Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic staining of glandular cells.

GW182 (C-19) - sc-47034 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GW182 (C-19): sc-47034. Western blot analysis of GW182 expression in non-transfected: sc-117752 (A) and human GW182 transfected: sc-113721 (B) 293T whole cell lysates.

GW182 (2D6) - sc-56313 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
GW182 (2D6): sc-56313. Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic and membrane staining of glandular cells.

GW182 (M-17) - sc-47036 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
GW182 (M-17): sc-47036. Western blot analysis of GW182 expression in non-transfected: sc-117752 (A) and human GW182 transfected: sc-113721 (B) 293T whole cell lysates.

GW182 (H-70) - sc-66915 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

Anti-GW182 antibody - ab84403 from Abcam

Prices $388.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
IHC image of ab84403 staining in Breast Cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84403, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-GW182 antibody - ab87541 from Abcam

Host Rabbit
Clonality Polyclonal

Anti-GW182 antibody - ab156173 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab156173 stained MCF7 cells. The cells were 100% methanol fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab156173, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa and HepG2 cells at 5µg/ml.

Anti-GW182 antibody - ab110233 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab110233 stained HeLa cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110233, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% paraformaldehyde fixed (10 min) MCF7 cells at 1µg/ml.

Anti-GW182 antibody - ab114857 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of GW182 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab114857 at 10 µg/mg lysate for IP (Lane 1). For WB detection an anti-GW182 antibody which recognizes a upstream epitope was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-GW182 antibody [4B6] - P/GW Body Marker - ab70522 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4B6
ab70522 staining GW182 in pre-fixed HEp-2000 cells. ab70522 was used at 1/100 dilution and incubated overnight at 4°C. An Alexa Fluor® 488-conjugated anti-mouse IgG was used as the secondary antibody.

Anti-TNRC6A / GW182 Antibody (aa1225-1275) - LS-C289081 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-TNRC6A / GW182 Antibody (N-Terminus) - LS-C101879 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
TNRC6A / GW182 antibody LS-C101879 Western blot of MCF7 cell lysate.

Anti-TNRC6A / GW182 Antibody (aa575-625) - LS-C289080 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human GW182 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-GW182 antibody used for IP at 6 ug/mg lysate. GW182 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated GW182, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

GW182 Antibody - NBP1-28751 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: GW182 Antibody [NBP1-28751] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GW182 antibody NBP1-28751 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 10 ug/mg lysate. GW182 was also immunoprecipitated by rabbit anti-GW182 antibody NBP1-28702, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

GW182 Antibody - NBP1-88232 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: GW182 Antibody [NBP1-88232] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG sp

GW182 Antibody - NBP1-57134 from Novus Biologicals

Prices $199.00, $369.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal

GW182 Antibody - NBP1-28702 from Novus Biologicals

Prices $369.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunoprecipitation: GW182 Antibody [NBP1-28702] - Immunoprecipitation on mouse brain samples - (dissected hippocampus). The recommended dilution is 1:100.

Anti-TNRC6A antibody produced in rabbit - HPA015305 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-TNRC6A: <B>Cat. No. HPA015305</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human testis shows strong nuclear positivity in cells of seminiferous ducts.

Anti-TNRC6A antibody produced in rabbit - HPA017869 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-TNRC6A: <B>Cat. No. HPA017869</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon shows strong cytoplasmic and nuclear positivity in glandular cells. Mesenchymal cells were distinctly stained.
Search more