ROS1 (D4D6 ® ) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 3287
• Prices: $129.00, $397.00
• Sizes: 20 µl (2 western blots), 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Applications: WB IP IHC-P ICC/IF FC
• Species Reactivities: Human
• Antigen: Monoclonal antibody is produced by immunizing animals with a protein corresponding to residues in the carboxy terminal domain of the human ROS1 protein.
• Description: Rabbit Monoclonal
• Gene: ROS1

Product images

Western blot analysis of extracts from HCC78 (SLC34A2-ROS1), U-118 MG (FIG-ROS1), and HeLa (ROS1 negative) cells using ROS1 (D4D6 ® ) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Note: HCC78 cells express the 85, 70, and 59 kDa forms of the SLC34A2-ROS1 fusion protein (7).

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing wildtype ROS1 (hROS1; +), using ROS1 (D4D6 ® ) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Immunoprecipitation of ROS1 from HCC78 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or ROS1 (D4D6 ® ) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using ROS1 (D4D6 ® ) Rabbit mAb, followed by Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 and Anti-mouse IgG, HRP-linked Antibody #7076.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using ROS1 (D4D6 ® ) Rabbit mAb. Note: Staining is of FIG-ROS1 fusion (6).

Immunohistochemical analysis of paraffin-embedded human lung carcinomas showing distinct localization using ROS1 (D4D6 ® ) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded HCC78 xenograft using ROS1 (D4D6 ® ) Rabbit mAb.

Confocal immunofluorescent analysis of HCC78 (left) or HeLa (right) cells using ROS1 (D4D6 ® ) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Flow cytometric analysis of HeLa (blue) and HCC78 (green) cells using ROS1 (D4D6 ® ) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.