Phospho-p90RSK (Ser380) (D5D8) Rabbit mAb

• Supplier: Cell Signaling Technology
• Catalog: 12032
• Prices: $287.00
• Sizes: 100 µl (10 western blots)
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: D5D8
• Applications: WB IP FC
• Species Reactivities: Human, Mouse, Rat, Monkey, Chicken, Xenopus, Zebrafish, Bovine, Dog, Pig, Horse
• Antigen: Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser377 of human p90RSK3 protein.
• Description: Rabbit Monoclonal
• Gene: RPS6KA1

Product images

Western blot analysis of extracts from various cell lines, starved overnight and untreated (-) or treated with either TPA #4174 (200 nM, 15 min; +), hEGF #8916 (100 ng/ml, 15 min; +), or hPDGF-BB #8912 (100 ng/ml, 15 min; +), using Phospho-p90RSK (Ser380) (D5D8) Rabbit mAb.

Immunoprecipitation of p90RSK from HeLa cell extracts, serum starved overnight and untreated (-) or treated with TPA #4174 (200 nM, 15 min; +), using Normal Rabbit IgG #2729 (lanes 5 and 6) or Phospho-p90RSK (Ser380) (D5D8) Rabbit mAb (lanes 3 and 4). Lanes 1 and 2 are 10% input. Western blot analysis was performed using Phospho-p90RSK (Ser380) Antibody #9341.

Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with TPA #4174 (green), using Phospho-p90RSK (Ser380) (D5D8) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) #4414 was used as a secondary antibody.