SIRT1 (B-7): sc-74465. Western blot analysis of SIRT1 expression in K-562 nuclear extract (A) and BJAB (B), MES-SA/Dx5 (C) and F9 (D) whole cell lysates.
SIRT1 (B-7): sc-74465. Western blot analysis of SIRT1 expression in non-transfected 293T: sc-117752 (A), human SIRT1 transfected 293T: sc-113797 (B) and F9 (C) whole cell lysates.
SIRT1 CRISPR/Cas9 KO Plasmid transfected: sc-400085. Western blot analysis of human SIRT1 CRISPR/Cas9 KO Plasmid expression in empty vector control (A) and SIRT1 CRISPR/Cas9 KO Plasmid transfected (B) 293T whole cell lysates. Blot probed with SIRT1 (B-7): sc-74465. α-actinin (H-12): sc-17829 used as specificity and loading control.