Formalin-fixed and paraffin-embedded human lung carcinoma reacted with U2AF1 Antibody (Center Y114) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of U2AF1 (arrow) using rabbit polyclonal U2AF1 Antibody (Center Y114). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the U2AF1 gene.
Western blot of anti-U2AF1 Antibody (Center Y114) in Ramos cell line lysates (35 ug/lane). U2AF1(arrow) was detected using the purified antibody.
U2AF1 Antibody (Center Y114) flow cytometry of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.