Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of UBE2I (arrow) using rabbit polyclonal UBE2I Antibody (S7). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the UBE2I gene.
Western blot of anti-UBE2I Antibody in A2058 cell line lysates (35 ug/lane). UBE2I(arrow) was detected using the purified antibody.