UBE2I

Gene Symbol UBE2I
Entrez Gene 7329
Alt Symbol C358B7.1, P18, UBC9
Species Human
Gene Type protein-coding
Description ubiquitin-conjugating enzyme E2I
Other Description SUMO-1-protein ligase|SUMO-conjugating enzyme UBC9|SUMO-protein ligase|ubiquitin carrier protein 9|ubiquitin carrier protein I|ubiquitin conjugating enzyme 9|ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)|ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)|ubiquitin-conjugating enzyme UbcE2A|ubiquitin-like protein SUMO-1 conjugating enzyme|ubiquitin-protein ligase E2I|ubiquitin-protein ligase I
Swissprots D3DU69 P63279 Q59GX1
Accessions AAK61274 CBJ93564 CBK25700 EAW85673 EAW85674 EAW85675 EAW85676 EAW85677 EAW85678 EAW85679 P63279 AB208988 BAD92225 AJ002385 CAA05359 AK024172 AK093616 AK291118 BAF83807 AL833737 BC000427 AAH00427 BC000744 BC004429 AAH04429 BC004437 BC014997 BC051289 AAH51289 BC064492 BC069256 BE262845 BF315618 BG530957 BI523501 BI793063 BQ230918 BQ358820 BT006932 AAP35578 BT007010 AAP35656 BU146357 BU543455 CD249028 CX788607 D45050 BAA08091 DQ891373 ABM82299 DQ894552 ABM85478 U29092 AAC51361 U31882 AAC50603 U31933 AAB02181 U38785 AAB09410 U45328 AAA86662 U66818 AAC50715 U66867 AAC50716 X96427 CAA65287 XM_005255540 XP_005255597 XM_005255541 XP_005255598 XM_011522644 XP_011520946 XM_011522645 XP_011520947 NM_003345 NP_003336 NM_194259 NP_919235 NM_194260 NP_919236 NM_194261 NP_919237
Function Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Can catalyze the formation of poly- SUMO chains. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation. Sumoylates p53/TP53 at 'Lys-386'. {ECO:0000269|PubMed:11451954, ECO:0000269|PubMed:15809060, ECO:0000269|PubMed:17466333, ECO:0000269|PubMed:19638400, ECO:0000269|PubMed:19744555, ECO:0000269|PubMed:20077568, ECO:0000269|PubMed:8668529}.
Subcellular Location Nucleus. Cytoplasm. Note=Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body (By similarity). {ECO:0000250}.
Tissue Specificity Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. {ECO:0000269|PubMed:8610150}.
Top Pathways Ubiquitin mediated proteolysis, RNA transport, MicroRNAs in cancer

Ubc9 (D26F2) XP ® Rabbit mAb - 4786 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D26F2
Western blot analysis of extracts from various cell lines using Ubc9 (D26F2) XP ® Rabbit mAb.

Ubc9 Antibody - 4918 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Ubc9 Antibody.

UBC9 (C-12) - sc-271057 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
UBC9 (C-12): sc-271057. Western blot analysis of UBC9 expression in Hep G2 (A), Jurkat (B) and HeLa (C) whole cell lysates.

UBC9 (H-81) - sc-10759 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
UBC9 (H-81): sc-10759. Immunoperoxidase staining of formalin fixed, paraffin-embedded human malignant lymphoma tissue showing nuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

UBC9 (N-15) - sc-5231 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
UBC9 (N-15): sc-5231. Immunofluorescence staining of methanol-fixed Jurkat cells showing nuclear staining.

UBC9 (67AT1273.95.90) - sc-130281 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
UBC9 (67AT1273.95.90): sc-130281. Western blot analysis of UBC9 expression in HL60 whole cell lysate.

UBC9 (50) - sc-136245 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
UBC9 (50): sc-136245. Western blot analysis of UBC9 expression in human endothelial tissue extract.

Anti-UBE2I / UBC9 antibody [1B10] - ab119958 from Abcam

Host Mouse
Clonality Monoclonal
Clone 1B10
Anti-UBE2I / UBC9 antibody [1B10] (ab119958) at 1/500 dilution + Recombinant UBE2I / UBC9 protein

Anti-UBE2I / UBC9 antibody [EP2938Y] - ChIP Grade - ab75854 from Abcam

Prices $404.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2938Y
All lanes : Anti-UBE2I / UBC9 antibody [EP2938Y] - ChIP Grade (ab75854) at 1/10000 dilutionLane 1 : U937 cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLane 4 : HUVEC cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/1000 dilution

Anti-UBE2I / UBC9 antibody [EP2938Y] - ChIP Grade (Alexa Fluor® 488) - ab198588 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2938Y
Overlay histogram showing HepG2 cells stained with ab198588 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab198588, 1/50 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-UBE2I / UBC9 antibody [EP2938Y] - ChIP Grade (Alexa Fluor® 647) - ab198589 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2938Y
ab198589 staining UBE2I / UBC9 in HepG2 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198589 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This product also gave a positive signal under the same testing conditions in HepG2 cells fixed with 100% methanol (5min).

Anti-UBE2I / UBC9 antibody - N-terminal - ab135110 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-UBE2I / UBC9 antibody - N-terminal (ab135110) at 1/1000 dilution + 293T total cell extract

Anti-UBE2I / UBC9 Antibody (Biotin) - LS-C210955 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-UBE2I / UBC9 Antibody (N-Terminus, clone EP2938Y) - LS-C105386 from LifeSpan Bioscience

Prices $505.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2938Y
IHC of paraffin-embedded human brain tissue using anti-UBC9 antibody (LS-C105386).

Anti-UBE2I / UBC9 Antibody - LS-C193100 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot.

Anti-UBE2I / UBC9 Antibody (FITC) - LS-C210956 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-UBE2I / UBC9 Antibody - LS-C210958 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-UBE2I / UBC9 Antibody (HRP) - LS-C210957 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-UBE2I / UBC9 Antibody (APC) - LS-C283933 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-UBE2I / UBC9 Antibody (Biotin) - LS-C283934 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-UBE2I / UBC9 Antibody (FITC) - LS-C283935 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-UBE2I / UBC9 Antibody (AP) - LS-C283936 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-UBE2I / UBC9 Antibody (Azide-free, HRP) - LS-C283937 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-UBE2I / UBC9 Antibody (PE) - LS-C283938 from LifeSpan Bioscience

Prices $515.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal

Anti-UBE2I / UBC9 Antibody (N-Terminus, APC) - LS-C283941 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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