SQSTM1 (D-3): sc-28359. Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
SQSTM1 (D-3): sc-28359. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
SQSTM1 (D-3): sc-28359. Western blot analysis of SQSTM1 expression in SK-LMS-1 (A) and HeLa (B) whole cell lysates.
SQSTM1 (D-3): sc-28359. Western blot analysis of SQSTM1 expression in HeLa whole cell lysate.
SQSTM1 (D-3): sc-28359. Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic and nuclear staining of glandular cells.
PSR HDR Plasmid (h): sc-400099-HDR. Western blot analysis of SQSTM1 expression in non-transfected control(E) and puromycin treated, SQSTM1 CRISPR/Cas9 KO Plasmid: sc-400099 plus PSR HDR Plasmid co-transfected (A-D) HEK 293T whole cell lysates. After puromycin selection, cells (A-D) were cloned by limiting dilution to isolate single cell colonies and then grown to confluency prior to lysis. Note the evidence of allelic effect: lysates B/C/D/E/F exhibit knockout of both alleles, resulting in 100% knockout of protein expression. Blot probed with SQSTM1 (D-3): sc-28359. β-Actin (C4): sc-47778 used as specificity and loading control.
PSR HDR Plasmid (h): sc-400099-HDR. Western blot analysis of SQSTM1 expression in non-transfected control(A) and puromycin treated, SQSTM1 CRISPR/Cas9 KO Plasmid: sc-400099 plus PSR HDR Plasmid co-transfected (B-F) HEK 293T whole cell lysates. After puromycin selection, cells (B-F) were cloned by limiting dilution to isolate single cell colonies and then grown to confluency prior to lysis. Note the evidence of allelic effect: lysates B/C/D/E/F exhibit knockout of both alleles, resulting in 100% knockout of protein expression. Blot probed with SQSTM1 (D-3): sc-28359. β-Actin (C4): sc-47778 used as specificity and loading control.
SQSTM1 CRISPR/CAS9 KO Plasmid (h): sc-400099. Western blot analysis of SQSTM1 expression in empty vector control (A) and SQSTM1 CRISPR/Cas9 KO Plasmid transfected (B) HEK293T whole cell lysates. Blot probed with SQSTM1 (D-3): sc-28359. β-actin (C4): sc-47778 used as specificity and loading control.