Anti-ULK1 Antibody (phospho-Ser556)
| Name | Anti-ULK1 Antibody (phospho-Ser556) |
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-C157021 |
| Prices | $295.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC-P WB |
| Species Reactivities | Human, Monkey |
| Purity/Format | Protein A purified |
| Blocking Peptide | ULBP1 Antibody Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | ULK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Fluorescent image of U251 cells stained with ULK1 (phospho S556) antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ULK1 (phospho S556) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ULK1 (phospho S556) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Dot blot of ULK1 Antibody (Phospho S556) Phospho-specific antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.