ULK1

Gene Symbol ULK1
Entrez Gene 8408
Alt Symbol ATG1, ATG1A, UNC51, Unc51.1, hATG1
Species Human
Gene Type protein-coding
Description unc-51 like autophagy activating kinase 1
Other Description ATG1 autophagy related 1 homolog|autophagy-related protein 1 homolog|serine/threonine-protein kinase ULK1
Swissprots O75385 Q9UQ28
Accessions CBX47626 EAW98529 O75385 AB018265 BAA34442 AF045458 AAC32326 AK095774 AK128333 BC111603 AAI11604 BI822269 BU729635 CF131673 XM_011538798 XP_011537100 XM_011538799 XP_011537101 XR_944753 NM_003565 NP_003556
Function Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. {ECO:0000269|PubMed:18936157, ECO:0000269|PubMed:21460634, ECO:0000269|PubMed:21795849}.
Subcellular Location Cytoplasm, cytosol {ECO:0000250}. Preautophagosomal structure {ECO:0000250}. Note=Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome. {ECO:0000250}.
Tissue Specificity Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
Top Pathways AMPK signaling pathway, Regulation of autophagy, mTOR signaling pathway

Phospho-ULK1 (Ser317) Antibody - 6887 from Cell Signaling Technology

Prices $279.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from 293T cells, mock transfected or transfected with a construct overexpressing mouse ULK1 using Phospho-ULK1 (Ser317) Antibody. Membranes were untreated (upper) or treated with Calf Intestinal Phosphatase (CIP) (lower) to demonstrate phospho-specificity.

Phospho-ULK1 (Ser757) Antibody - 6888 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from A172 cells, untreated (-), Torin 1-treated (+; 250 nM; 5 hrs), or INK-128-treated (+; 250 nM; 5 hrs) using Phospho-ULK1 (Ser757) Antibody (upper) or total ULK1 (D8H5) Rabbit mAb #8054 (lower).

Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb - 5869 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1H4
Western blot analysis of extracts from MCF7 cells, untreated or treated with oligomycin #9996 (0.5 μM, 30 minutes), and C2C12 cells, untreated or treated with hydrogen peroxide (10 mM, 5 minutes), using Phospho-ULK1 (Ser555) (D1H4) Rabbit mAb.

Phospho-ULK1 (Ser467) Antibody - 4634 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from mock transfected and mouse ULK1 (mULK1) transfected 293T cells, untreated or treated with λ-phosphatase and calf intestinal phosphatase (CIP), using Phospho-ULK1 (Ser467) Antibody (upper) or ULK1 (R600) Antibody #4773 (lower).

Phospho-ULK1 (Ser317) (D2B6Y) Rabbit mAb - 12753 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D2B6Y
Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with carbonyl cyanide 3-chlorophenylhydrazone (CCCP) (100 μM, 2 hr; +), using Phospho-ULK1 (Ser317) (D2B6Y) Rabbit mAb (upper) or ULK1 (D8H5) Rabbit mAb #8054 (lower).

Phospho-ULK1 (Ser638) (D8K9O) Rabbit mAb - 14205 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D8K9O
Western blot analysis of extracts from A172 cells, untreated (-), Torin 1-treated (250 nM; 5 hr; +), or INK128-treated (250 nM; 5 hr; +) using Phospho-ULK1 (Ser638) (D8K9O) Rabbit mAb (upper) or ULK1 (D8H5) Rabbit mAb #8054 (lower).

Phospho-ULK1 (Ser757) (D7O6U) Rabbit mAb - 14202 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D7O6U
Western blot analysis of extract from A172 cells, untreated (-) or treated with mTOR inhibitors, either Torin-1 (250 nM, 5 hrs), Torin-2 (250 nM, 5 hrs), or INK128 (250 nM, 5 hours) using Phospho-ULK1 (Ser757) (D7O6U) Rabbit mAb (upper) or ULK1 (D8H5) Rabbit mAb #8054 (lower).

ULK1 (D9D7) Rabbit mAb - 6439 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D9D7
Western blot analysis of extracts from various cell lines using ULK1 (D9D7) Rabbit mAb.

ULK1 (A705) Antibody - 4776 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS cells, untransfected (-) or transfected with a construct overexpressing human ULK1 (+), using ULK1 (A705) Antibody.

ULK1 (R600) Antibody - 4773 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS cells, untransfected (-) or transfected with a construct overexpressing human ULK1 (+), using ULK1 (R600) Antibody.

ULK1 (D8H5) Rabbit mAb - 8054 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D8H5
Western blot analysis of extracts from various cell lines using ULK1 (D8H5) Rabbit mAb.

Phospho-ULK1 (Ser638) Antibody - 12097 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

ULK1 (F-4) - sc-390904 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ULK1 (F-4): sc-390904. Western blot analysis of ULK1 expression in non-transfected: sc-117752 (A) and human ULK1 transfected: sc-158046 (B) 293T whole cell lysates.

ULK1 (H-240) - sc-33182 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
ULK1 (H-240): sc-33182. Western blot analysis of ULK1 expression in non-transfected 293T: sc-117752 (A), human ULK1 transfected 293T: sc-158046 (B) and Jurkat (C) whole cell lysates.

ULK1 (N-17) - sc-10900 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

ULK1 (E-20) - sc-10902 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ULK1 (E-20): sc-10902. Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.

Anti-ULK1 (phospho S450) antibody [EPR6152] - ab155971 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6152
All lanes : Anti-ULK1 (phospho S450) antibody [EPR6152] (ab155971) at 1/1000 dilutionLane 1 : 293T cell lysate transfected with ULK1.Lane 2 : 293T cell lysate transfected with ULK1, treated with Lambda PhosphataseLysates/proteins at 10 µg per lane.

Anti-ULK1 (phospho S467) antibody [EPR6153(2)] - ab177472 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6153(2)
All lanes : Anti-ULK1 (phospho S467) antibody [EPR6153(2)] (ab177472) at 1/10000 dilutionLane 1 : 293T cell lysateLane 2 : ULK1 transfected 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat-anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Anti-ULK1 (phospho S467) antibody [EPR6153(2)-118] - ab179458 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6153(2)-118
All lanes : Anti-ULK1 (phospho S467) antibody [EPR6153(2)-118] (ab179458) at 1/1000 dilutionLane 1 : Lysate from 293T cells transfected with ULK1. Membrane was not treated with Lambda Phosphatase.Lane 2 : Lysate from 293T cells transfected with ULK1. Membrane was treated with Lambda Phosphatase.Lysates/proteins at 10 µg per lane.SecondaryStandard HRP labeled goat anti-rabbit at 1/2000 dilutiondeveloped using the ECL technique

Anti-ULK1 (phospho S556) antibody - ab203207 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed and paraffin embedded Rat brain tissue labeling ULK1 (phospho S556) using ab203207 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Anti-ULK1 (phospho S556) antibody [EPR6156] - ab133747 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6156
All lanes : Anti-ULK1 (phospho S556) antibody [EPR6156] (ab133747) at 1/10000 dilutionLane 1 : ULK1 transfected 293T cell lysate untreatedLane 2 : ULK1 transfected 293T cell lysate treated with Lambda PhosphataseLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-ULK1 (phospho S623) antibody [EPR6154] - ab131210 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6154
All lanes : Anti-ULK1 (phospho S623) antibody [EPR6154] (ab131210) at 1/1000 dilutionLane 1 : ULK1 transfected 293T cell lysate - untreatedLane 2 : ULK1 transfected 293T cell lysate - treated with Lambda phosphataseLysates/proteins at 10 µg per lane.

Anti-ULK1 (phospho S638) antibody [EPR6155] - ab133766 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR6155
All lanes : Anti-ULK1 (phospho S638) antibody [EPR6155] (ab133766) at 1/1000 dilutionLane 1 : ULK1 transfected 293T cell lysateLane 2 : ULK1 transfected 293T cell lysate, treated with Lambda PhosphataseLysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Anti-ULK1 (phospho S757) antibody - ab156920 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-ULK1 (phospho S757) antibody (ab156920) at 1 µg/mlLane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : EGF-Stimulated A431 Whole Cell LysateLane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 4 : EGF-Stimulated A431 Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Control peptide at 1 µg/mlLane 6 : EGF-Stimulated A431 Whole Cell Lysate with Control peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-ULK1 antibody - ab65056 from Abcam

Host Rabbit
Clonality Polyclonal
Ab65056 staining human normal renal medulla. Staining is localized to subcellular compartment.Left panel: with primary antibody at 1/2000. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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