Anti-ULK1 Antibody (aa642-672)
| Name | Anti-ULK1 Antibody (aa642-672) |
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-C156402 |
| Prices | $295.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC-P WB |
| Species Reactivities | Human, Monkey |
| Purity/Format | Protein A purified |
| Blocking Peptide | ULBP5 / RAET1G Antibody Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | ULK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Fluorescent image of U251 cells stained with APG1 (ULK1) antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated APG1 (ULK1) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). APG1 (ULK1) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
Western blot of anti-ULK1 antibody in A2058 cell lysate. ULK1 (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.