Detection of Human VAP-33 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-VAP-33 antibody used for IP at 6 ug per reaction. VAP-33 was also immunoprecipitated by rabbit anti-VAP-33 antibody BL16444. For blotting immunoprecipitated VAP-33, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human and Mouse VAP-33 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN or RIPA buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-VAP-33 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.