VAPA

Gene Symbol VAPA
Entrez Gene 9218
Alt Symbol VAP-33, VAP-A, VAP33, hVAP-33
Species Human
Gene Type protein-coding
Description VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
Other Description 33 kDa VAMP-associated protein|VAMP-A|VAMP-associated protein A|vesicle-associated membrane protein-associated protein A
Swissprots Q9P0L0 Q5U0E7 O75453 Q9UBZ2 D3DUI3 A6NDZ0
Accessions EAX01590 EAX01591 EAX01592 Q9P0L0 AF044670 AAD09742 AF057358 AAC26508 AF086627 AAD13576 AF154847 AAF72105 AK024299 AK096868 AK123434 AK292948 BAF85637 AK315577 BAG37951 BC002992 AAH02992 BC033947 BC042860 BG488667 BG719406 BP209270 BT019618 AAV38424 XM_011525769 XP_011524071 NM_003574 NP_003565 NM_194434 NP_919415
Function May play a role in vesicle trafficking. {ECO:0000269|PubMed:11511104, ECO:0000269|PubMed:19289470}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000269|PubMed:10523508, ECO:0000269|PubMed:19289470}; Single-pass type IV membrane protein {ECO:0000269|PubMed:10523508, ECO:0000269|PubMed:19289470}. Note=Present in the plasma membrane and in intracellular vesicles, together with SNARE proteins. May also associate with the cytoskeleton. Colocalizes with OCLN at the tight junction in polarized epithelial cells.
Tissue Specificity Ubiquitous.
Top Pathways Tight junction

VAP-A (H-40) - sc-98890 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
VAP-A (H-40): sc-98890. Western blot analysis of VAP-A expression in non-transfected: sc-117752 (A) and mouse VAP-A transfected: sc-124538 (B) 293T whole cell lysates.

VAP-A (K-14) - sc-48697 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VAP-A (K-14): sc-48697. Western blot analysis of VAP-A expression in non-transfected: sc-117752 (A) and mouse VAP-A transfected: sc-124538 (B) 293T whole cell lysates.

VAP-A (K-15) - sc-48698 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VAP-A (K-15): sc-48698. Western blot analysis of VAP-A expression in MDCK (A), HeLa (B) and NIH/3T3 (C) whole cell lysates.

VAP-A (N-16) - sc-48699 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VAP-A (N-16): sc-48699. Western blot analysis of VAP-A expression in non-transfected: sc-117750 (A), mouse VAP-A transfected: sc-124538 (B), HEK293 (C) and Hep G2 (D) whole cell lysates.

Anti-VAPA antibody - ab77002 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-VAPA antibody (ab77002) at 1 µg/ml + Recombinant tagged VAPA protein at 0.1 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Anti-VAPA antibody - C-terminal - ab174196 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-VAPA antibody - C-terminal (ab174196) at 1/100 dilution + K562 cell line lysates at 35 µg

Anti-VAP33 / VAPA Antibody (aa182-210, Azide-free, HRP) - LS-C247215 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VAP33 / VAPA Antibody (aa1-249, Biotin) - LS-C300766 from LifeSpan Bioscience

Prices $435.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-VAP33 / VAPA Antibody (aa1-249, FITC) - LS-C303695 from LifeSpan Bioscience

Prices $455.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-VAP33 / VAPA Antibody (C-Terminus) - LS-C187127 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot of Human PANC1. VAPA antibody dilution 1.0 ug/ml.

Anti-VAP33 / VAPA Antibody (aa182-210) - LS-C326264 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VAP33 / VAPA Antibody (aa182-210) - LS-C157030 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
VAPA Antibody western blot of K562 cell line lysates (35 ug/lane). The VAPA antibody detected the VAPA protein (arrow).

Anti-VAP33 / VAPA Antibody (aa1-249) - LS-C296980 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant VAP33 / VAPA.

Anti-VAP33 / VAPA Antibody (aa125-175) - LS-C291005 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human VAP-33 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-VAP-33 antibody used for IP at 6 ug per reaction. VAP-33 was also immunoprecipitated by rabbit anti-VAP-33 antibody BL16444. For blotting immunoprecipitated VAP-33, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Anti-VAP33 / VAPA Antibody - LS-C109464 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
IHC of paraffin-embedded lung AdCA, using VAPA antibody at 1:500 dilution.

Anti-VAP33 / VAPA Antibody (aa1-132) - LS-C132853 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-VAP33 / VAPA Antibody (aa182-210, AP) - LS-C242546 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VAP33 / VAPA Antibody (aa182-210, Biotin) - LS-C242548 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VAP33 / VAPA Antibody (aa182-210, APC) - LS-C242547 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VAP33 / VAPA Antibody (aa182-210, FITC) - LS-C242550 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-VAP33 / VAPA Antibody (aa182-210, PE) - LS-C242551 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

VAP-A Antibody - NBP1-31237 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: VAP-A Antibody [NBP1-31237] - Sample (30 ug of whole cell lysate) A:H1299 12% SDS PAGE, antibody diluted at 1:1000.

VAP-A Antibody (604101) - MAB5820 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 604101
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human VAP-A Monoclonal Antibody (Catalog # MAB5820) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for VAP-A at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 with 0.05% Tween 20.

VAP-A Antibody [Unconjugated] - AF5820 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal

VAP-A Antibody - NBP1-83132 from Novus Biologicals

Prices $129.00, $419.00
Sizes 25 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: VAP-A Antibody [NBP1-83132] - Analysis in human cell line MOLT-4.
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