Immunofluorescence of monoclonal antibody to VRK1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot analysis of VRK1 expression in transfected 293T cell line by VRK1 monoclonal antibody (M02), clone 4F9.Lane 1: VRK1 transfected lysate(45.5 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation of VRK1 transfected lysate using anti-VRK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with VRK1 monoclonal antibody.
Detection limit for recombinant GST tagged VRK1 is approximately 0.1 ng/ml as a capture antibody.
Western blot analysis of VRK1 over-expressed 293 cell line, cotransfected with VRK1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with VRK1 monoclonal antibody (M02) clone 4F9 . GAPDH ( 36.1 kDa ) used as specificity and loading control.