Western blot analysis of extracts from HT-29 cells, untreated (-) or treated with thymidine (2 mM, 16 hr) followed by Nocodazole #2190 (10 nM, 24 hr; +), using Securin (D2B6O) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human PTTG1 (securin) protein (hPTTG1-Myc/DDK; +) or Myc/DDK-tagged full-length human PTTG2 (securin 2) protein (hPTTG2-Myc/DDK; +), using Securin (D2B6O) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).
Immunoprecipitation of securin from Jurkat cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Securin (D2B6O) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Securin (D2B6O) Rabbit mAb.