Anti-Vimentin Antibody (aa430-457)
| Name | Anti-Vimentin Antibody (aa430-457) |
|---|---|
| Supplier | LifeSpan Bioscience |
| Catalog | LS-C98613 |
| Prices | $295.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC-P |
| Species Reactivities | Human, Mouse, Rat, Dog |
| Purity/Format | Ammonium sulfate precipitation |
| Blocking Peptide | Villin-Like / VILL Antibody Blocking Peptide |
| Description | Rabbit Polyclonal |
| Gene | VIM |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Vimentin Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human colon tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Fluorescent confocal image of SY5Y cells stained with Vimentin antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated Vimentin primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Vimentin immunosignal to the cytoskeleton, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000026025).
Western blot of Vimentin antibody in NCI-H460 cell line lysates (35 ug/lane). Vimentin (arrow) was detected using the purified antibody.
Vimentin Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.