VIM

Gene Symbol VIM
Entrez Gene 7431
Alt Symbol CTRCT30, HEL113
Species Human
Gene Type protein-coding
Description vimentin
Other Description epididymis luminal protein 113
Swissprots Q15869 Q6LER9 B0YJC2 P08670 Q96ML2 Q8N850 Q9NTM3 D3DRU4 Q548L2 Q15868 Q15867
Accessions AAA61279 AAA61280 AAA61281 ACA06100 ACA06101 ACA06102 ACA06103 CAA36187 EAW86215 EAW86216 P08670 AF328728 AAN09720 AK056766 BAB71275 AK091813 BAG52420 AK093924 AK097336 BAC05002 AK098444 AK222482 BAD96202 AK222507 BAD96227 AK222602 BAD96322 AK290643 BAF83332 BC000163 AAH00163 BC030573 AAH30573 BC031031 BC066956 AAH66956 BQ050765 CR407690 CAG28618 EU794672 ACJ13726 M25246 AAA61282 X16478 CAA34499 X56134 CAA39600 Z19554 CAA79613 XM_006717500 XP_006717563 XM_011519649 XP_011517951 NM_003380 NP_003371
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. {ECO:0000269|PubMed:21746880}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:21465480}.
Tissue Specificity Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines. {ECO:0000269|PubMed:2472876, ECO:0000269|PubMed:3371665}.
Top Pathways Epstein-Barr virus infection, MicroRNAs in cancer

Vimentin (D21H3) XP ® Rabbit mAb (HRP Conjugate) - 12826 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D21H3
Western blot analysis of extracts from NIH/3T3 and HeLa cells using Vimentin (D21H3) XP ® Rabbit mAb (HRP Conjugate).

Vimentin (D21H3) XP ® Rabbit mAb (Alexa Fluor ® 594 Conjugate) - 7675 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D21H3
Immunofluorescent analysis of SNB19 (left) and MCF7 (right) cells using Vimentin (D21H3) XP ® Rabbit mAb (Alexa Fluor ® 594 Conjugate) (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Vimentin (D21H3) XP ® Rabbit mAb (PE Conjugate) - 12020 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D21H3
Flow cytometric analysis of MCF7 (blue) and HeLa (green) cells using Vimentin (D21H3) XP ® Rabbit mAb (PE Conjugate).

Vimentin (D21H3) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) - 9856 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D21H3
Confocal immunofluorescent analysis of HeLa cells using Vimentin (D21H3) XP ® Rabbit mAb (Alexa Fluor ® 647 Conjugate) (blue pseudocolor). Red = Propidium Iodide (fluorescent DNA dye)

Vimentin (D21H3) XP ® Rabbit mAb (Alexa Fluor ® 555 Conjugate) - 9855 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D21H3
Confocal immunofluorescent analysis of SNB19 (left) or MCF7 (right) cells using Vimentin (D21H3) XP ® Rabbit mAb (Alexa Fluor ® 555 Conjugate) (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Vimentin (D21H3) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) - 9854 from Cell Signaling Technology

Prices $329.00
Sizes 100 µl (50 tests)
Host Rabbit
Clonality Monoclonal
Clone D21H3
Confocal immunofluorescent analysis of SNB19 cells using Vimentin (D21H3) XP ® Rabbit mAb (Alexa Fluor ® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® #4084 (fluorescent DNA dye).

Phospho-Vimentin (Ser56) Antibody - 3877 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types, hydroxyurea-treated (4 mM) (G1/S) or paclitaxel-treated (100 nM) (G2/M) for 20 hours, using Phospho-Vimentin (Ser56) Antibody (upper). β-Actin Antibody #4967 was used as a loading control (lower).

Phospho-Vimentin (Ser56) (D5H2) Rabbit mAb - 7391 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5H2
Western blot analysis of extracts from HeLa and C2C12 cells, hydroxyurea-treated (4 mM, G1/S arrested) or treated with Paclitaxel #9807 (100 nM, G2/M arrested) for 20 hr, using Phospho-Vimentin (Ser56) (D5H2) Rabbit mAb (upper) and Vimentin (D21H3) XP ® Rabbit mAb #5741 (lower).

Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb - 12569 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5A2D
Western blot analysis of extracts from HeLa cells, untreated (-), or treated with either hydroxyurea (4 mM, G1/S arrested; +), or Paclitaxel #9807 (100 nM, G2/M arrested; +) for 20 hr, using Phospho-Vimentin (Ser83) (D5A2D) Rabbit mAb (upper) and Vimentin (D21H3) XP ® Rabbit mAb #5741 (lower).

Phospho-Vimentin (Ser83) Antibody - 3878 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HeLa cells, untreated or phosphorylated in vitro by PLK, using Phospho-Vimentin (Ser83) Antibody (upper). β-Actin Antibody #4976 (lower) was used as a loading control.

Phospho-Vimentin (Ser39) Antibody - 13614 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Phospho-Vimentin (Ser39) Antibody (upper), Vimentin (D21H3) XP ® Rabbit mAb #5741 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower). Publications have shown that the human breast carcinoma cell line, ZR-75-1, does not express vimentin.

Vimentin (D21H3) XP ® Rabbit mAb - 5741 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D21H3
Western blot analysis of extracts from various cell lines using Vimentin (D21H3) XP ® Rabbit mAb.

Vimentin (R28) Antibody - 3932 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using Vimentin (R28) Antibody.

Vimentin (5G3F10) Mouse mAb - 3390 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 5G3F10
Western blot analysis of extracts from various cell types using Vimentin (5G3F10) Mouse mAb.

Vimentin Antibody - 3295 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

Vimentin (E-5) - sc-373717 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Vimentin (E-5): sc-373717. Western blot analysis of Vimentin expression in KNRK whole cell lysate.

Vimentin (H-84) - sc-5565 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Vimentin (H-84): sc-5565. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Vimentin (J144) - sc-53464 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Vimentin (J144): sc-53464. Western blot analysis of Vimentin expression in SJRH30 (A) and RD (B) whole cell lysates.

Vimentin (RV202) - sc-32322 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Vimentin (RV202): sc-32322. Western blot analysis of Vimentin expression in U-698-M whole cell lysate.

Vimentin (RV203) - sc-58899 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Vimentin (RV203): sc-58899. Western blot analysis of Vimentin expression in HeLa whole cell lysate.

Vimentin (S-20) - sc-7558 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Vimentin (S-20): sc-7558. Western blot analysis of Vimentin expression in HISM whole cell lysate.

Vimentin (V9) - sc-6260 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Vimentin (V9): sc-6260. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.

Vimentin (C-20) - sc-7557 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Vimentin (C-20): sc-7557. Western blot analysis of Vimentin expression in SJRH30 (A) and RD (B) whole cell lysates.

Vimentin (9E7E7) - sc-66001 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Vimentin (9E7E7): sc-66001. Western blot analysis of Vimentin expression in SJRH30 (A) and RD (B) whole cell lysates.

Vimentin (3CB2) - sc-80975 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Vimentin (3CB2): sc-80975. Western blot analysis of Vimentin expression in detergent-insoluble extracts from human U-87 glioma cells. Kindly provided by Dr. Enrique J. de la Rosa, Consejo Superior de Investigaciones Cientficas.
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