Vimentin Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human prostate tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot of Vimentin Antibody in A2058,A375 cell line lysates (35 ug/lane). Vimentin (arrow) was detected using the purified antibody.
Western blot of Vimentin (arrow) using Vimentin Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the VIM gene (Lane 2) (Origene Technologies).
Vimentin Antibody flow cytometry of HeLa cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.