Western blot analysis of extracts from COS cells transfected with SGK1 using Phospho-SGK1 (Ser78) (D36D11) Rabbit mAb, untreated or following antibody pre-incubation with either a site-specific phosphorylated peptide to block the signal or a site-specific non-phosphorylated peptide that cannot block the signal.
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with H 2 O 2 (4 mM for 15 min), using Phospho-SGK1 (Ser78) (D36D11) (upper) or SGK1 Antibody #3272 (lower).
Western blot analysis of extracts from COS cells, untransfected (-) or transfected with human SGK1 (+), using Phospho-SGK1 (Ser78) (D36D11) (upper) or SGK1 Antibody #3272 (lower).