ZIC1 in Mouse Brain. ZIC1 was detected in immersion fixed frozen sections of mouse brain (cerebellum) using 15 ug/ml Goat Anti-Human/Mouse ZIC1 Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Tissue was stained with Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue).
Detection of Human/Mouse ZIC1 by Western Blot. Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human/Mouse ZIC1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for ZIC1 at approximately 54 kD (as indicated). This experiment was conducted under reducing conditions.