Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing either DDK-tagged full-length human SHMT1 (hSHMT1-DDK; +) or DDK-tagged full-length human SHMT2 (hSHMT2-DDK; +), using SHMT1 Antibody (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Immunoprecipitation of SHMT1 from extracts of HeLa cells using Normal Rabbit IgG #2729 (lane 2) or SHMT1 Antibody (lane 3). Lane 1 is 10% input. Western blot analysis was performed using SHMT1 Antibody. Mouse Anti-rabbit IgG (Light-Chain Specific) (L57A3) mAb #3677 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as secondary antibodies.