Western blot analysis of extracts from various cell lines using SIRPα/SHPS1 (D6I3M) Rabbit mAb (upper) and GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using SIRPα/SHPS1 (D6I3M) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lymph node using SIRPα/SHPS1 (D6I3M) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded ACHN (left) or MCF7 (right) cell pellets using SIRPα/SHPS1 (D6I3M) Rabbit mAb.
Confocal immunofluorescent analysis of ACHN (positive, left) and MCF7 (low expression, right) cells, using SIRPα/SHPS1 (D6I3M) Rabbit mAb (green). Blue pseudocolor= DRAQ5 ® #4084 (fluorescent DNA dye).
Flow cytometric analysis of Jurkat cells (blue) and U937 cells (green) using SIRPα/SHPS1 (D6I3M) Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) #4412 was used as a secondary antibody.