SIN3A (D9D6) Rabbit mAb
| Name | SIN3A (D9D6) Rabbit mAb |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 8056 |
| Prices | $246.00 |
| Sizes | 100 µl (10 western blots) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | D9D6 |
| Applications | WB IP ICC/IF ChIP |
| Species Reactivities | Human, Monkey |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu530 of human SIN3A protein. |
| Description | Rabbit Monoclonal |
| Gene | SIN3A |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from various cell lines using SIN3A (D9D6) Rabbit mAb.
Confocal immunofluorescent analysis of 293 cells using SIN3A (D9D6) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 MCF7 cells grown in phenol red free medium and charcoal stripped FBS for 4 days then treated with β-estradiol (10 nM) for 1 hr and either 10 μl of SIN3A (D9D6) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human ESR1 Promoter Primers #9673, SimpleChIP ® Human GAPDH Exon 1 Primers #5516, SimpleChIP ® Human MyoD Exon 1 Primers #4490, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.