Western blot analysis of extracts from SirT1 wild-type (WT) and knockout (KO) mouse embryo fibroblasts (MEF) using SirT1 (1F3) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Wild-type and knockout MEFs were a gift from Wenyi Wei at the Harvard Medical School.
Western blot analysis of extracts from various cell lines using SirT1 (1F3) Mouse mAb.
Confocal immunofluorescent analysis of MEF WT (left, positive) and MEF SirT1 KO (right, negative) cells using
SirT1 (1F3) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Wild-type and knockout MEFs were a gift from Wenyi Wei at the Harvard Medical School.