Western blot analysis of extracts from various cell lines using SirT1 (D1D7) Rabbit mAb.
Western blot analysis of extracts from SirT1 wild-type (WT) and knockout (KO) mouse embryonic fibroblasts (MEF) using SirT1 (D1D7) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). WT and KO MEF were kindly provided by Wenyi Wei, Harvard Medical School.
Confocal immunofluorescent analysis of HeLa (positive; left), WT MEF (positive; middle), and SirT1 KO MEF (right) cells using SirT1 (D1D7) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). WT and KO MEF were kindly provided by Wenyi Wei, Harvard Medical School.